: Section-Cutting A Practical Guide to the Preparation and Mounting of Sections for the Microscope Special Prominence Being given to the Subject of Animal Sections by Marsh Sylvester - Microscopy; Microscopy Technique

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Introduction 11 On Cutting Unprepared Vegetable Tissues 12 On Cutting Unprepared Animal Tissues 13 Preparation of Vegetable Tissues 15 Preparation of Animal Tissues 16 Special Methods of Hardening 19 Cutting Hardened Tissues by Hand 19 Microtome 20 AEther Microtome 24 Section-Knife 24 Imbedding in Paraffine for Microtome 26 Employment of Microtome 29 Staining Agents 31 Carmine Staining 32 Mounting Media 36 Mounting in Glycerine 38 Uses of Freezing Microtome 42 Employment of Freezing Microtome 43 Logwood Staining 47 Absolute Alcohol 50 Clove Oil 51 Canada Balsam 51 Mounting in Balsam 53 Finishing the Slide 55

Special Methods 57 Bone 57 Brain 59 Cartilage 60 Coffee Berry 62 Fat 63 Hair 63 Horn, etc. 64 Intestine 64 Liver 65 Lung 65 Muscle 66 Orange-peel 67 Ovary 67 Porcupine Quill 67 Potato 67 Rush 68 Skin 68 Spinal Cord 69 Sponge 71 Stomach 71 Tongue 71 Vegetable Ivory 72 Wood 72 Note A, 75 Note B, 75 Note C, 76 Note D, 76 Note E, 76 Note F, 77 Note G, 77 Note H, 93 Note I, 94 Index

SECTION-CUTTING.

It is evident, therefore, that nothing can be done with such refractory materials until, by subjection to appropriate methods of preparation, they shall have been reduced to such a consistence as to render them suitable for cutting. How this is to be accomplished will depend entirely upon the physical and chemical nature of the substance to be operated upon. As the various objects differ so widely from each other in these respects, so must the methods of preparation suitable to each also vary. It is clear, therefore, that no general directions for attaining this end can be given which would be of any practical value. It is possible, however, and very convenient, to arrange the various objects into groups or classes, to the treatment of each of which certain general rules are applicable; but there will still remain a comparatively numerous series of objects whose individual peculiarities of structure will demand for them correspondingly special methods of preparation. When such objects come to be spoken of, the particular treatment most suitable to each will also be noticed.

Footnote 1:

Note A.

Footnote 2:

Note B.

Footnote 3:

Note C.

Footnote 4:

Note D.

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